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OBJECTIVE:To compare the effects of Qili qiangxin capsules and Compound danshen drop pills on related indexes of acute myocardial infarction(AMI)patients without reflow after percutaneous coronary intervention(PCI). METHODS:A total of 90 AMI patients without reflow after PCI were randomly divided into Compound danshen drop pills group (45 cases) and Qili qiangxin capsules group(45 case). Both groups were given Aspirin chewable tablets 300 mg and Clopidogrel sulfate tablets 300 mg before PCI. After surgery,both groups were given Aspirin chewable tablets 100 mg,once a day+simvastatin chewable tablets 10 mg,once a day. Based on it,Compound danshen drop pills group was given Compound danshen drop pills,10 pills,3 times a day. Qili qiangxin capsules group was given Qili qiangxin capsules,4 capsules,3 times a day. Both groups were treated for consec-utive 6 months. The blood flow classification of TIMI,LEVF,β1-AR,β2-AR,α1-AR,bleeding and the occurrence of major cardio-vascular events were observed in 2 groups before and after treatment. The occurrence of ADR was recorded. RESULTS:Bafore treatment,there was no statistical significance in the blood flow classification of TIMI,the levels of β1-AR,β2-AR and α1-AR be-tween 2 groups(P>0.05). After treatment,the case number of 0-2 degree blood flow classification of TIMI in 2 groups were signif-icantly lower than before treatment while the case number of 3 degree were significantly higer than before treatment;the levels ofβ1-AR,β2-AR and α1-AR were significantly lower than before treatment,while LEVF was significantly higher than before treatment;LEVF of Qili qiangxin capsules group was significantly higher than that of Compound danshen drop pills group,with statistical signifi-cance (P0.05). No obvious ADR was found in 2 groups during treatment. CONCLUSIONS:Qili qiangxin capsules is similar to Com-pound danshen drop pills in improving no-reflow efficacy and safety of AMI patients after PCI,but it is better than Compound dan-shen drop pill in improving cardiac function.
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Objective To observe the effect of ephedrine on the expression of eotaxin in human bronchial epithelial cells (16HBE) stimulated by tumor necrosis factor‐α(TNF‐α) and to explore the mechanism of Chinese medicine ephedra in treating asthma .Methods The in vitro cultured 16HBE were randomly divided into the control group ,TNF‐αstimulation group(TNF‐α20 ng/mL) and TNF‐αplus ephedrine group (TNF‐α20 ng/mL plus ephedrine 300 μg/mL) .Three complex holes in each group were set to culture for 18 h ,the eotaxin mRNA expression was measured by real time fluorescent quantified PCR and protein level was detected by immunocytochemical stain and Western blot .The eotaxin concentration in cells culture supernatant was quantified by ELISA .Results Compared with the the control group ,the expression level of eotaxin mRNA and protein ,and the concentration of eotaxin in cell culture supernatant in the TNF‐α stimulation group were increased obviously ,there being statisticaly significant difference between them(P<0 .01);however ,all above these parameters in the TNF‐αplus ephedrine group showed decreased obvi‐ously as compared with the TNF‐αgroup ,the difference between them was statistically significant (P<0 .01) .Conclusion Ephed‐rine can inhibit the expression and secretion of eotaxin in TNF‐α induced 16HBE inflammatory model ,which may be one of the mechanisms of Chinese medicine ephedra in treating asthma .
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Objective To study the relationship between the number of outpatient complaints and the time or the outpatient amounts.Methods A retrospective analysis of 1289 cases of outpatients' medical complaints with basic records since 2009 in our hospital was carried out.The time regularity of the number of outpatient complaints,or the complaint rate was analyzed.And the correlation between the outpatient amounts and the number of outpatient complaints,or the complaint rate was also analyzed.Results In our hospital,the mean monthly number of outpatient complaints was (23.44±8.41)cases; the mean monthly outpatient account was (246 000±27 417)cases.The differences among monthly outpatient complaints,outpatient accounts and monthly complaints rate among every years were statistically significant.Within one year,the outpatient complaints numbers were lowest in February and January.And the outpatient complaints rate was low in November,January,February and December.There were statistically significant correlation between the monthly outpatient complaints and monthly outpatient accounts,and between the monthly outpatient complaints rate and monthly outpatient accounts.Conclusions There was time regularity in the outpatient complaints rates in one year.And there was certain correlation between the outpatient complaints rate and the outpatients' accounts.
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BACKGROUND:At present,syndromes happening frequency of diabetes mellitus can be controlled through regulating glucose concentration in blood.Therefore,a fast,steady,accurate glucose biosensor has been an intense area of focus for investigators.OBJECTIVE:To observe the effect of chitosan-gold nanoparticles hybrid film on electrochemical glucose biosensor.DESIGN,TIME AND SETTING:The observation experiment was performed at the Department of Medicine Laboratory,General Hospital,Guangzhou Military Command of Chinese PLA from February to April 2009.MATERIALS:Based on the immobilization of glucose oxidase (GOD) with cross-linking in the matrix of chitosan on an Au electrode by glutaraldehyde,a novel glucose biosensor was constructed with gold nanoparticles by electrodeposition.METHODS:The properties of CHIT/AuNPs hybrid film modified electrode were characterized by cyclic voltammetry (CV) in 20 mmol/L K3Fe (CN)6-0.1 mol/L KCI system by using the three-electrode system and the response to glucose was investigated by linear scan voltammetry (LSV).The optimal experiment conditions,stability and reproducibility about the glucose biosensor were also studied.MAIN OUTCOME MEASURES:The cyclic voltammetry properties of different modified electrode,as well as relation between glucose concentration and response current.RESULTS:Glucose concentration showed a linear correlation with response current in range of 0.001-6 mmol/L.The biosensor exhibited excellent performances of producibility,stability and the ignorance interferences of reducing substances,with the CONCLUSION:The glucose biosensor possesses excellent reproducibility and stability with a fast response time to glucose,which can be used in detection of glucose concentration in human serum.
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To investigate the effect of arginine vasopression (AVP) on the secretion of vascular endothelial growth factor (VEGF) by vascular endothelial cell (VEC). VEC of human greater omentum artery were isolated and cultured. The concentrations of VEGF and activity of protein kinase C (PKC) in the culture medium were measured. Results indicated that after the cells were incubated with 1?10 -9～1?10 -7mol/L AVP for 6 hours, the concentration of VEGF and activity of PKC were markedly increased compared to control (P
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Objective To construct a kind of novel electrochemical glucose biosensor for clinical detection.Methods Multiwall carbon tube(MWCNTs),NanoAu and glucose oxidase(GOD) were immobilized on the Au electrode by monolayer self-assemble.Cyclic voltammetry and linear scan voltammetry were used to investigate the assembling process and the chemical characteristics of the glucose biosensor.Results The linearity of glucose in the range of 10～1 400 mg/L with the correlation coefficient of R2=0.990 9 was obtained by linear scan voltammetry and the detection limited reached 0.000 1 mg/L.The four criterion samples were measured with 3 different biosensors in same conditions and the RSD